primary rabbit anti-human egfr antibody  (Biogenex)

Journal: Cancer immunology research

Article Title: C3aR Signaling Inhibits NK-cell Infiltration into the Tumor Microenvironment in Mouse Models

doi: 10.1158/2326-6066.CIR-21-0435

Figure Lengend Snippet: a. Immunoprecipitation to assess interaction between C3aR and CD11a (αL subunit that is specific to LFA-1)(n=2). b, c. Representative CD11a staining in activated human NK cells with or without C3a stimulation (n=3). d, e. Representative LFA-1 epitope 24 staining in activated human NK cells with or without C3a stimulation (n=3). f, g. Phospo-CD18 (T758) staining with or without C3a stimulation (n=3). Scale bar: 50 µm. Boxed areas indicate area shown in zoomed images.

Article Snippet: Primary rabbit anti-human CD11a (Cat#ab52895, Abcam, USA), primary rabbit anti-human CD18 (phospo-T758; Cat#ab63388, Abcam, USA), primary mouse anti-human CD11a+CD18 antibody [24] (Cat# ab13219, Abcam, USA), or respective control antibody (Rabbit IgG and Mouse IgG1) was diluted as suggested by the provider, infused into the microfluidic devices, and incubated for overnight at 4 o C. Samples were then washed three times and incubated with diluted secondary antibody (1:200 dilution; anti-mouse goat IgG, Cat# A32723 or anti-rabbit goat IgG, Cat# A32721, Thermofisher Scientific, USA) in the dark at room temperature for one hour.

Techniques: Immunoprecipitation, Staining

Journal: Oncotarget

Article Title: Biomarker quantification by multiplexed quantum dot technology for predicting lymph node metastasis and prognosis in head and neck cancer

doi: 10.18632/oncotarget.9225

Figure Lengend Snippet: ( A ) library composed of DAPI, auto-fluorescence, QD565, QD625, QD655, and QD705 was initially set for the analysis. Signals in the image cube were unmixed according to their wavelengths in the library and then the corresponding signals were separated. E-cad is shown in cyan, EGFR in red, cytoplasmic vimentin in yellow, and β-actin in green. A: The expression of three biomarkers in primary tumors from patients with or without LNM. ( B ) Quantum dot library (normalized spectrum). C-F: segmentation of cancer cell from stroma ( C ), nucleus ( D ), cytoplasm ( E ), and membrane ( F ).

Article Snippet: The immunoreaction sequences were: 1) primary rabbit anti-human EGFR antibody (Biogenex, Fremont, CA), overnight at 4°C; secondary goat anti-rabbit conjugated-QD705 (Invitrogen, Carlsbad, CA), at 37°C, 2 hour.

Techniques: Fluorescence, Expressing, Membrane